Hoyos Carlos

Proyectos en ejecución:
  • Integrante del proyecto PICT-2014-1579. Consejo Nacional de Investigaciones Científicas y Técnicas. Tema: Estrategias noveles de ADN recombinante aplicadas al inmunodiagnóstico de las leishmaniasis endémicas en Argentina. Desde 2015 al 2018.
  • Integrante del proyecto del CIUNSa N° 1990 “Leishmaniasis Tegumentaria: Eficacia de los tratamientos de Leishmaniasis efectuados en Orán, en el período 1998-2008”. Desde el 01 de Enero de 2012 al 31 de Diciembre de 2015.
Información general (Experticia en CyT):
Lic. en Ciencias Biológicas. Fac. Cs. Naturales. Universidad Nacional de Salta (Año 2012). Participa en proyectos de investigación relacionados con el diagnóstico serológico, molecular  y epidemiología de enfermedades, particularmente leishmaniasis. Tiene experiencia en desarrollo de trabajos a campo para muestreos de insectos y micromamíferos; y de laboratorio para aislamiento, cultivo, crio-preservación y tipificación Leishmania sp.
 
Publicaciones:
  1. Epidemiology of American Tegumentary Leishmaniasis and Trypanosoma cruzi Infection in the Northwestern Argentina. Hoyos CL, Cajal SP, Juárez M, Marco JD, Alberti D’Amato AM, Cayo M, Torrejón I, Cimino RO, Diosque P, Krolewiecki AJ, Nasser JR y Gil JF. BioMed Research International. 2016, Article ID 6456031.
 
Presentaciones a congresos:
  1. Hoyos CL, Uncos A, Cajal SP, Caro N, Juárez M, Almazán MC, Bracamonte ME, Moya Alvarez, A, Gil, JF, Nasser JR, Krolewiecki A y MarcoJD. El desempeño de diferentes métodos de PCRs en el diagnóstico de Leishmaniasis Tegumentaria Americana. XXVIII Reunión Anual de la Sociedad Argentina de Protozoología y Enfermedades Parasitarias. Libro de resúmenes: 88; DyT99. Santa Fe, Noviembre  2016.
  2. Hoyos CL, Cajal P, Juarez M, Barroso PA, Bracamonte ME, Portelli S, Gil JF, Nasser JR, Krolewiecki AJ, Ruybal P, Marco JD. Estrategias para el diagnóstico de leishmaniasis tegumentaria americana (LTA) por PCR en el Norte de Salta. X Congreso de Protozoología y enfermedades parasitarias. Libro de Resúmenes: 126; DyT51. Mar del Plata, Noviembre 2014.
  3. Aramayo L, Copa N, Hoyos C, Villalpando C, Cajal P, Juárez M, Avila M, Acosta N, Carlos M, Krolewiecki A, Abán Y, Corro E, Sosa N, Aparicio JP, Nasser JR, Gil JF. VI Jornadas de Comunicaciones y IV Jornadas de Enseñanza de las Ciencias Naturales en Salta. Infección por Trypanosoma cruzi en el Departamento Orán, Salta. Fac. Cs. Naturales-UNSa, Salta. Noviembre 2013.
  4. Hoyos CL, Barroso PA, Locatelli FM, Cardozo RM, Mora MC, García Bustos MF Bracamonte E, Uncos A, Ramos F, Gentile AG, Barrio AB, Korenaga M, Hashiguchi Y, Basombrío MA, Marco JD. Leishmaniosis canina y fauna flebotominea en sitios rurales del noroeste de Salta. XXVI Reunión Anual de la Sociedad Argentina de Protozoología. Libro de Resúmenes: 92. Rosario, Octubre 2013.
Mail: 
carloslhoyos@gmail.com
 
Posición:
  • Becario doctoral CONICET.
Línea de investigación (breve):
  • Evaluación de sistemas de marcadores moleculares: como complemento de métodos diagnósticos convencionales, para tipificación de especies de Leishmania aisladas de pacientes y para la detección de infección natural por Leishmania sp. en micromamíferos silvestres, potenciales reservorios de leishmaniasis en el norte de Salta.
Fecha: 
25/09/2018
Linea de Investigacion: 

Cutaneous leishmaniasis is endemic in Salta province, which belongs to the northwest of Argentina. Leishmania spp. DNA from Giemsa-stained slides of up to 12 years in storage of patients from Salta was characterized through PCR-restriction fragment length polymorphism. One hundred smears positive for microscopy, classified in a semiquantitative scale for amastigote density, were analyzed. Also, Leishmanin skin test (LST) results were included. DNA extraction was carried out applying lysis buffer with proteinase K, and then DNA was amplified with ribosomal internal transcribed spacer 1 primers. PCR products were digested with HaeIII enzyme. All PCR-positive smears (74/100) belonged to Viannia subgenus. A statistically significant, directly proportional relationship between semiquantitative microscopy and PCR results was detected. All patients had LST-positive results (induration ≥ 5 mm), and the smears of those with smaller induration (LST < 19 mm) gave a higher proportion of positive PCR results. This study determined that smear age did not affect PCR positivity, which allows retrospective analyzes and suggests smears might be useful for molecular complementary diagnosis. Because Leishmania (Viannia) braziliensis is the main circulating species in the study area, determining Viannia subgenus in all analyzed samples confirms previous findings. PCR positivity showed statistically significant differences according to semiquantitative microscopy, highlighting the importance of parasite burden in the diagnostic sensitivity of the method. Considering that smears of patients with smaller LST induration were more positive in PCR, a negative smear from patients with positive LST response, but < 19 mm, could actually represent a false-negative result.

 

© The American Society of Tropical Medicine and Hygiene

Fecha: 
03/08/2018
Linea de Investigacion: 

Abstract

The sand fly fauna in Hipólito Yrigoyen, Argentina, a locality where cutaneous leishmaniasis cases occur, was surveyed with zones of higher abundance of sand flies correlated to vegetation cover estimated through normalized difference vegetation index (NDVI). Sand flies were collected with 10 CDC traps during six nights, from December 2009 to January 2010. A map was built of expected sand flies abundance in which levels of NDVI were categorized. In total, 1,392 Phlebotominae (Diptera: Psychodidae) specimens were collected, comprised of the following species: Nyssomyia neivai (Pinto 1926), Migonemyia migonei (França 1920), species of the cortelezzii complex (Brèthes 1923), Evandromyia sallesi (Galvão & Coutinho 1940), and Psathyromyia shannoni (Dyar 1929). Positive correlations were found between the abundance of sand flies and the NDVI (P < 0.05) for buffer areas of <150 m radii from the trap location points, i.e., the sand fly abundance was greater where vegetation cover and density were greater. In this context, plant cover should be taken into account to prioritize surveillance and control areas within the program of sand flies control in northern Argentina.