Almazán María Cristina

Información general (Experticia en CyT):
Soy licenciada en Ciencias Biológicas de la Universidad Nacional de Salta. Estoy haciendo el doctorado en Cs. Biológicas en la Facultad de Ciencias Naturales de la UNSa. Soy docente de la Cátedra de Zoología en la misma universidad. Actualmente realizó búsqueda de infección natural de Leishmania sp.  en flebótomos mediante disección. Desarrollo también, técnicas moleculares como PCR y RFLP para tipificación de flebótomos y parásitos.
 
Presentaciones a Congresos:
  • “SAND FLIES CAPTURES AND IDENTIFICATION OF LEISHMANIA SUBGENUS IN GIEMSA-STAINED SLIDES OF PATIENTS FROM FIVE LOCALITIES OF SALTA, ARGENTINA” Almazán María Cristina, Gil José F, Chanampa Mariana M , Barroso Paola A, Copa Griselda N, Oreste F, Castillo G, Nasser Julio R. VIII International Symposium on Plebotominae. Puerto Iguazú, Septiembre 2014.
  • “ANALISIS RESTROSPECTIVO DE FROTIS DE PACIENTES DEL NORTE DE SALTA, MEDIANTE PCR-RFLP, PARA LA IDENTIFICACIÓN DE SUBGÉNEROS DE LEISHMANIA”  Almazán MC, Gil JF, Marco JD, Cajal SP, Portelli M, Juarez M, Krolewiecki AJ, Barroso P, Hoyos CL, Nasser JR. X Congreso de Protozoología y Enfermedades Parasitarias. Sociedad Argentina de Protozoología. Buenos Aires, Noviembre 2014.
  •  “TIPIFICACIÓN MOLECULAR DE LEBÓTOMOS DE UN ÁREA ENDÉMICA DE LEISHMANIASIS TEGUMENTARIA EN LA PROVINCIA DE SALTA, MEDIANTE PCR-RFLP DEL GEN ARNr 18S” María C Almazán, Griselda N Copa, Inés R López Quiroga, Carlos L Hoyos, José F Gil, Jorge D Marco, Julio R Nasser, Paola A Barroso. XXVIII Reunión Anual de la Sociedad Argentina de Protozoología y Enfermedades Parasitarias. Santa Fe, Noviembre de 2016.

Otros datos: Docente de la Universidad Nacional de Salta.

Mail: 
cristina.almazan90@gmail.com
 
Posiciones:
  • Becaria Doctoral de CONICET. Docente de la Universidad Nacional de Salta.
Línea de investigación (breve):
  • Identificación de vectores de leishmaniasis en la provincia de Salta.
Proyecto de tesis doctoral:
“Determinación de las especies de Lutzomyia que actúan como vectores de Leishmania (Viannia) braziliensis, principal agente causal de la leishmaniasis en la Provincia de Salta”.
Fecha: 
16/11/2018
Linea de Investigacion: 

Abstract

Background

Some sand flies are of medical importance because they are vectors of Leishmania parasites that are responsible for leishmaniasis. The aim of this study was to make a retrospective epidemiological analysis of tegumentary leishmaniasis (TL), to identify Leishmania spp. from patient isolates and to describe the diversity of sand flies from a border area between Bolivia and Argentina.

Methods

TL cases included in the study were diagnosed in an endemic area of the north of Argentina from 1985 to 2017. The parasites isolated were characterized by the cytochrome B method. Sandflies were captured with Centers for Disease Control traps in Aguas Blancas and Media Luna-Algarrobito localities.

Results

A total of 118 cases of TL were analysed. Eight isolates were characterized as Leishmania (Viannia) braziliensis. A total of 1291 sandflies were captured, including Nyssomyia neivai, Cortelezzii complex, Evandromyia sallesiMigonemyia migonei and Micropygomyia quinquefer. Within the area, sandflies were found in the backyards of houses.

Conclusions

In this region there exists the possibility of peridomestic transmission of TL in the neighbourhoods peripheral to the urban area and in rural environments as well as the risk of transmission to travellers that pass through the customs offices.

Fecha: 
25/09/2018
Linea de Investigacion: 

Cutaneous leishmaniasis is endemic in Salta province, which belongs to the northwest of Argentina. Leishmania spp. DNA from Giemsa-stained slides of up to 12 years in storage of patients from Salta was characterized through PCR-restriction fragment length polymorphism. One hundred smears positive for microscopy, classified in a semiquantitative scale for amastigote density, were analyzed. Also, Leishmanin skin test (LST) results were included. DNA extraction was carried out applying lysis buffer with proteinase K, and then DNA was amplified with ribosomal internal transcribed spacer 1 primers. PCR products were digested with HaeIII enzyme. All PCR-positive smears (74/100) belonged to Viannia subgenus. A statistically significant, directly proportional relationship between semiquantitative microscopy and PCR results was detected. All patients had LST-positive results (induration ≥ 5 mm), and the smears of those with smaller induration (LST < 19 mm) gave a higher proportion of positive PCR results. This study determined that smear age did not affect PCR positivity, which allows retrospective analyzes and suggests smears might be useful for molecular complementary diagnosis. Because Leishmania (Viannia) braziliensis is the main circulating species in the study area, determining Viannia subgenus in all analyzed samples confirms previous findings. PCR positivity showed statistically significant differences according to semiquantitative microscopy, highlighting the importance of parasite burden in the diagnostic sensitivity of the method. Considering that smears of patients with smaller LST induration were more positive in PCR, a negative smear from patients with positive LST response, but < 19 mm, could actually represent a false-negative result.

 

© The American Society of Tropical Medicine and Hygiene