Fecha: 
25/09/2018
Linea de Investigacion: 

Cutaneous leishmaniasis is endemic in Salta province, which belongs to the northwest of Argentina. Leishmania spp. DNA from Giemsa-stained slides of up to 12 years in storage of patients from Salta was characterized through PCR-restriction fragment length polymorphism. One hundred smears positive for microscopy, classified in a semiquantitative scale for amastigote density, were analyzed. Also, Leishmanin skin test (LST) results were included. DNA extraction was carried out applying lysis buffer with proteinase K, and then DNA was amplified with ribosomal internal transcribed spacer 1 primers. PCR products were digested with HaeIII enzyme. All PCR-positive smears (74/100) belonged to Viannia subgenus. A statistically significant, directly proportional relationship between semiquantitative microscopy and PCR results was detected. All patients had LST-positive results (induration ≥ 5 mm), and the smears of those with smaller induration (LST < 19 mm) gave a higher proportion of positive PCR results. This study determined that smear age did not affect PCR positivity, which allows retrospective analyzes and suggests smears might be useful for molecular complementary diagnosis. Because Leishmania (Viannia) braziliensis is the main circulating species in the study area, determining Viannia subgenus in all analyzed samples confirms previous findings. PCR positivity showed statistically significant differences according to semiquantitative microscopy, highlighting the importance of parasite burden in the diagnostic sensitivity of the method. Considering that smears of patients with smaller LST induration were more positive in PCR, a negative smear from patients with positive LST response, but < 19 mm, could actually represent a false-negative result.

 

© The American Society of Tropical Medicine and Hygiene

Comunicado

  • Posted on: 6 September 2018
  • By: admin_iiet

  El Instituto de Investigaciones de Enfermedades Tropicales expresa su preocupación y rechazo a la pérdida de los Ministerios de Salud y de Ciencia y Tecnología. Reafirmando nuestra vocación y compromiso a seguir trabajando en los ejes científico y sanitario, vemos y queremos alertar acerca de estas recientes decisiones del Gobierno Nacional como un retroceso dañino y peligroso; por eso pedimos reconsiderar estas medidas.

Manual de Técnicas básicas para diagnóstico parasitológico

  • Posted on: 30 August 2018
  • By: admin_iiet

PROTOCOLO DE TÉCNICAS DE DIAGNÓSTICO PARASITOLÓGICO

TÉCNICA DE CONCENTRACIÓN

Materiales:

  • Frasco de toma de muestra de materia fecal
  • Gasa cuádruple
  • Embudo
  • Centrífuga
  • Tubo de centrífuga de plástico de 15 ml
  • Palillos de plástico
  • Portaobjetos
  • Cubreobjetos
  • Colorante Amido Schwartz (ver anexo para preparación de este colorante).
  • Varilla de madera o de vidrio

 

Páginas