José Fernando Gil

Date: 
25/09/2018
Investigation Line: 

Cutaneous leishmaniasis is endemic in Salta province, which belongs to the northwest of Argentina. Leishmania spp. DNA from Giemsa-stained slides of up to 12 years in storage of patients from Salta was characterized through PCR-restriction fragment length polymorphism. One hundred smears positive for microscopy, classified in a semiquantitative scale for amastigote density, were analyzed. Also, Leishmanin skin test (LST) results were included. DNA extraction was carried out applying lysis buffer with proteinase K, and then DNA was amplified with ribosomal internal transcribed spacer 1 primers. PCR products were digested with HaeIII enzyme. All PCR-positive smears (74/100) belonged to Viannia subgenus. A statistically significant, directly proportional relationship between semiquantitative microscopy and PCR results was detected. All patients had LST-positive results (induration ≥ 5 mm), and the smears of those with smaller induration (LST < 19 mm) gave a higher proportion of positive PCR results. This study determined that smear age did not affect PCR positivity, which allows retrospective analyzes and suggests smears might be useful for molecular complementary diagnosis. Because Leishmania (Viannia) braziliensis is the main circulating species in the study area, determining Viannia subgenus in all analyzed samples confirms previous findings. PCR positivity showed statistically significant differences according to semiquantitative microscopy, highlighting the importance of parasite burden in the diagnostic sensitivity of the method. Considering that smears of patients with smaller LST induration were more positive in PCR, a negative smear from patients with positive LST response, but < 19 mm, could actually represent a false-negative result.

 

© The American Society of Tropical Medicine and Hygiene

Date: 
01/06/2016
Investigation Line: 
Abstract
Resumen: Los eventos de salud y sus determinantes varían en el espacio y el tiempo. Poder conocer los patrones espacio-temporales, representa un objetivo primordial de la epidemiología como herramienta de la salud pública. Este conocimiento es el sustrato mediante el cual se pueden generar programas o intervenciones preventivas y de control. En la provincia de Salta, el sistema sanitario ha avanzado considerablemente en cuanto a la informatización y manejo en red de la información recabada por la Atención Primaria de la Salud en áreas operativas, sub áreas y/o sectores sanitarios. El presente trabajo plantea y fundamenta la necesidad de consensuar con diferentes sectores (principalmente el INDEC) unidades básicas de recolección de datos que permitan el "cruce" de información tanto para la investigación por parte del personal de Salud, como de instituciones científicas bajo convenio con el Ministerio de Salud. En este sentido se plantea la generación de una IDE en salud pública de la provincia de Salta considerando dos usos principales: a) uso interno del sistema de salud e instituciones científicas y b) servicios online públicos como potenciales estrategias de prevención de enfermedades. Se abordan dos ejemplo, uno referido a Aedes aegypti, dengue, chikungunya y zika y otro referido a la leishmaniasis.